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Original Research Article | OPEN ACCESS

MiR-214 promotes renal fibrosis in diabetic nephropathy via targeting SOCS1

Weiwei Xi1, Xuming Zhao1, Meijun Wu2, Xueqin Fu1, Wenjuan Jia1, Mingxi Lu1, Hua Li1

For correspondence:-  Hua Li   Email: h_li@zju.edu.cn   Tel:+8657186006875

Accepted: 25 April 2019        Published: 31 May 2019

Citation: Xi W, Zhao X, Wu M, Fu X, Jia W, Lu M, et al. MiR-214 promotes renal fibrosis in diabetic nephropathy via targeting SOCS1. Trop J Pharm Res 2019; 18(5):1009-1015 doi: 10.4314/tjpr.v18i5.14

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To elucidate how miR-214 regulates the pathogenesis of diabetic nephropathy (DN).
Methods: The extent of fibrosis in DN mice kidneys was examined using Masson’s staining. Quantitative polymerase chain reaction (qPCR) was used to determine the levels of miR-214. Dual luciferase reporter assay was used to identify the target of miR-214. The expression of fibrosis marker proteins of high glucose-stimulated NRK-52E cells transfected with miR-214 was determined using western blotting.
Results: Fibrosis in renal tissue of DN mice was significantly increased and miR-214 was upregulated (p < 0.001). Suppressor of cytokine signaling 1 protein (SOCS1) was the target gene of miR-214, and overexpression of miR-214 promoted fibrosis (p < 0.05, p < 0.001). On the other hand, overexpression of SOCS1 inhibited this process, indicating that miR-214 promoted fibrosis via targeting SOCS1 (p < 0.001). Finally, inhibition of miR-214 c ameliorated renal fibrosis in DN mice (p < 0.01, p < 0.001).
Conclusions: MiR-214 is upregulated in db/db DN mice kidney tissue; miR-214 regulates renal fibrosis in DN mice by targeting SOCS1.

Keywords: MiR-214, Renal fibrosis, Suppressor of cytokine signaling 1

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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